Silicosen

Silicosen and Bio-Silico are air-permeable silicone rubber plugs for tubes and erlenmeyer flasks for the cultivation of micro-organisms, yeasts, molds and others. The plugs have continuous pores giving them excellent performance and properties as substitute for conventional cotton plugs. The water evaporation rate is significantly lower which avoids cultivars to dry and concentrations to change thus making culture tests more reliable and improving repeatability of tests.

Features of Silicosen and Bio-Silico:
  • Silicosen and Bio-Silico are handled more easily than conventional cotton plugs.
  • Silicosen and Bio-Silico are suitable for the cultivation of aerobic micro-organisms.
  • Bio-Silico is optimal for shaking cultures.
  • Silcosen has excellent heat resistance and is reusable by dry air sterilization at 180ºC or autoclaving.
  • Silicosen can withstand a temperature of 350ºC for a short time allowing flame sterilization.
  • Silicosen can withstand a temperature of 200ºC for 10000 hours.
  • The water evaporation rate through Silicosen is less than half of the rate through a cotton plug by which culture media will not dry or change concentrations or pH level.
  • Silicosen and Bio-Silico have excellent chemical resistance.
  • Silicosen and Bio-Silico prevent the stray entry of airborne bacteria for both stationary and shaking cultures.
  • Silicosen and Bio-Silico can be cleaned with hot water and neutral detergents.
  • Due to its hydrophobic property, Silicosen prevents microbes from proliferating on its surface even at high atmospheric moisture levels.
 
Types of Silicosen
 Plug Type
 (T-  and L-types)
 
 
 This popular type reduces water evaporation
 in a way drying culture medium can be
 diminished. Various sizes are available to
 fit test tubes for a wide range of applications.
   
 Cap Type
 (C-type)


 This type is designed to be easily fitted and
 detached. Furthermore cultivars in shaken
 flasks or tubes will not touch the cap easily
 due to the thin layer of Silicosen in the cap.
 
 Super Type
 (S-type)


 This type of plug is also designed for shaken
 cultivars due to the thin layer of Silicosen at
 the top of the plug. The cultivar will not touch
 the Silicosen material easily.
 
 Bio-Silico
 (N-type)



 This type has excellent air-permeability due
 to the open cell structures at the side of the plug
 Various sizes are available to fit test tubes for a
 wide range of applications.
 
Basic properties
   Unit       Reference
 plug
 Air permeability  cc/min  T-type
 C-type
 S-type
 N-type
 Cotton plug (reference)
 Paper plug (reference)
     30 ~ 120
 2000 ~ 4500
 2500 ~ 4500
 3800 ~ 4500
     50 ~ 2000
   200 ~ 1500
 T-42
 C-55
 S-40
 N-42
 -
 -
 Test method: use a flowmeter to measure flowrate with a water column of 26 mm. Flask used is a 500 cc conical flask.

 

Evaporation of water
   
 Moisture evaporation rate is half or less of cotton plug for T-types and approximately the same as cotton
 plug for C-types.
 Test method: inject 5 ml of water into a medium sized test tube, seal tube with culture plag and leave
 standing. the fluctuation in weight due to moisture evaporation is measured with elapse of time.

 

Sterilization Methods
   Silicosen  Bio-Silico
 High-pressure steam sterilization (120ºC / 20 ~ 30 min)  very good  very good
 Dry air sterilization (120ºC / 20 ~ 30 min)  good  good
 Dry air sterilization (180ºC / 20 ~ 30 min)  good  not possible
 Flame sterilization  short time  not possible

 

Chemical resistance - tensile strength
   Chemicals  Silicosen  Bio-Silico
Acids  3% hydrochloric acid  10 ~ 25 %  25 ~ 75 %
 10% sulfuric acid  < 10 %  25 ~ 75 %
 7% nitric acid  25 ~ 75 %  > 75 %
 5% acetic acid  10 ~ 25 %  < 10 %
 Bases
 Salts
 Others
 20% NaOH  10 ~ 25 %  > 75 %
 20% sodium carbonate  10 ~ 25 %  < 10 %
 10% sodium chloride  < 10 %  < 10 %
 10% aquaous ammonia  10 ~ 25 %  10 ~ 25 %
 3% hydrogen peroxide  < 10 %  10 ~ 25 %
 23ºC water  < 10 %  < 10 %
 100ºC water *  10 ~ 25 %  < 10 %
 Oils  vegetable oil  10 ~ 25 %  < 10 %
 silicone oil  10 ~ 25 %  10 ~ 25 %
 Solvents  ethyl alcohol  25 ~ 75 %  10 ~ 25 %
 acetone  25 ~ 75 %  10 ~ 25 %
 carbon tetrachloride  > 75 %  25 ~ 75 %
 toluene  > 75 %  25 ~ 75 %
 neutral detergent  < 10 %  < 10 %
 Test method: immerse and leave for one week in various tpes of chemicals at room temperature (23ºC) and then observe and evaluate the tensile strength changes.
 * 100ºC x 1 hr

Note:
Every item or numerical value indicated herein is measured by Shin-Etsu and out of guarantee.
The quality of the product is out of guarantee.
Please make sure to review the purpose and conditions of use and practice you own tests.